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Muscles were prepared as described in "Muscle preparation" above.
In the histological preparation above, a newly hatched little skate, Leucoraja erinacea, was specially stained: blue specifically stains cartilage whilst red stains minerals.
The SVZ from YFP mice was co-cultivated in contact with wild type mouse cortex (see organotypic preparation above).
Bone marrow was extracted from femurs at each time point as described under the section on neonatal femur preparation above.
Labels of "'C' above the amino acid sequence show locations of carbamidomethyl modification (a result of chemical alkylation directed at cysteines during sample preparation), 'O' above the amino acid sequence show locations of oxidation (spontaneous oxidation of methionine), and 'P' above the amino acid sequence show locations of phosphorylation.
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Following termination of fermentation and extract preparation using above mentioned protocol, TLC profiles of the both the extracts were compared in mobile phase containing 5% MeOH in DCM.
For further sample preparation, the above protocol was followed.
In contrast to the indices of global task preparation analyzed above, our measures of motor-specific preparation showed no switch-related asymmetries across the 2 tasks.
For immunofluorescence, following initial preparation as above, tissues were blocked with NGS (Biosera) diluted 1 5 with 5% (w/ v) BSA in TBS (NGS/TBS/BSA) before incubation with 5mC antibody (1 100, mouse, Eurogentec) overnight at 4°C.
Following tissue preparation as above, tissues were blocked with NGS/TBS/BSA and incubated with either 5fC or 5caC antibodies (1 200 or 1 1,500, respectively, both Active Motif) overnight at 4°C.
We ended up with 13 decoys that showed a Δdocking rank between the two preparations above the cutoff.
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CEO of Professional Science Editing for Scientists @ prosciediting.com