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"He took our level of preparation a step higher.
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Ms. Cotter has taken her preparations a step further, however.
For interaction studies with cell membrane preparations, a step gradient of 10, 20, 30, and 70% sucrose was used.
For large-scale preparations, a step gradient was applied with a third extensive washing step containing 60 mM imidazole and an elution step with 250 mM imidazole yielding in 300 μg of highly purified ATPase protein (from 1g (wet weight) of cells).
His preparation for a step was designed to signal something remarkable coming: he deliberately created tension, as Kavanagh says, in order to release it in virtuosity.
Automated sample preparation incorporated a step to calculate the volume of sample collection based on sample weight and solution density of 1 g/mL.
So instead he envisaged an evolution in food-preparation technology, a step beyond the rolling hot-dog cart, because without the necessary permits, neither the grill nor the sausages could touch the ground.
However, in order to exclude any differences in background phosphorylation between enzyme preparations a dephosphorylation step was included before activation experiments.
Therefore, standard QTL mapping codes normally perform a preparation step by evaluating a ij x) at regularly spaced grid points in the genome.
In a final preparation step a further silica sol gel layer was deposited.
This approach saves a preparation step for the reverse transcription (e.g., polyA-tailing, ligation of a 3′-adapter) and allows the direct accessibility of the 3′-fragment ends as the sequence used in the stem of the loop adapter is identical to the Illumina Paired-Read Primer 2. V) Several size selection steps are performed to remove adapter dimers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com