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In addition to optimization of sample preparation, a quantitative strategy to identify DEPs was employed using the mass spectrum data.
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Second, while immunohistochemistry is an in situ technique in which data are obtained subjectively, RT PCR is performed on disaggregated tissue preparations in a quantitative manner.
To determine the mitochondrial DNA copy number fragment in the preparation we performed a quantitative PCR (qPCR), as described in Ermini et al. [24].
In short, both the small and the large-scale native purification methods are competitive with a conventional denaturing technique for a quantitative preparation of RNA.
A mouse monoclonal antibody WF6 was raised against a shark cartilage aggrecan preparation [ 8], and a quantitative ELISA for the epitope recognized by monoclonal antibody WF6 was modified from a previous study [ 9].
To provide practically operational feasibility for the preparation of a predicted flocculant, a quantitative mathematical correlation between the preparation recipe and the structural features of SCPAMPAA were derived.
The microfluidic platform provides a quantitative sample preparation and analysis method at the cell level that could be widely applied in the environmental and energy fields.
Although differences in BSEP/Bsep staining intensity were observed between vesicle preparations of the different species, a quantitative estimate of transport protein abundance could not be made.
Using a radioiodinated antibody preparation and quantitative biodistribution studies in tumor-bearing mice, AP2 was shown to selectively localize to fibrin-rich F9 murine teratocarcinomas, but not to SKRC-52 human kidney cancer xenografts.
Without the complication of ocular optics, isolated retinas can provide a simple ex vivo preparation for quantitative analysis of the contribution of individual retinal layers/cells, including the photoreceptor and inner retinal neurons, to the fundus autofluorescence.
The collection of data concerning sage preparations should be expanded in the future, preferably compounded by a quantitative risk-benefit analysis.
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