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The premium select fetal bovine serum (FBS) was obtained from Atlanta Biologicals (Lawrenceville, GA).
hMSC were grown in MEM alpha media supplemented with antibiotic-antimycotic and 10% premium select FBS.
HEK 293T cells ATCC® CRL-11268™ were cultured in Dulbecco's Modified Eagle Medium (D-MEM) high glucose (Invitrogen, Carlsbad, CA) supplemented with 10% (v/v) heat-inactivated Fetal Bovine Serum (FBS) Premium Select, Lot # C0136 (Atlanta Biologicals, Lawrenceville, GA) and 1% penicillin-streptomycin (HyClone, Logan, UT) at 37°C in a humidified incubator containing 5% CO2.
MSCs were maintained as subconfluent monolayers in basic medium [Dulbecco's Modified Eagle Medium (DMEM; GIBCO-BRL, Gaithersburg, MD) containing 10% calf bovine serum (Premium Select; Atlanta Biologicals, Atlanta, GA), 100 IU/mL penicillin, 100 μg/mL streptomycin, and 1 mM sodium pyruvate] as described previously (Kirchner et al. 2010).
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3T3-L1 pre-adipocytes were cultured in high glucose-L-glutamine DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% Fetal Bovine Serum-Premium Select (Atlanta Biologicals, Lawrenceville, GA) at 37°C with 5% CO2.
NIH3T3 fibroblasts were cultured in high glucose-L-glutamine DMEM (Invitrogen, Carlsbad, CA) with 10% Fetal Bovine Serum-Premium Select (Atlanta Biologicals, Lawrenceville, GA) at 37°C with 5% CO2.
Premium was selected as a "neutral facility," said Mr. Olsen-Harbich.
This limited-edition gift set features two premium wines selected from the Chef Andrés' cellars in Spain, in a wooden box signed by chef José Andrés and artist Sami Hayek, a copy of his Made in Spain cookbook and his famed "serving shoe". The glass shoe, modeled after Converse's iconic Chuck Taylors, is designed and created by artist Sami Hayek.
The Outnet's editorial team carefully consider each product that is pitched to us and we place a premium on selecting the right merchandise versus offering a mass quantity of bargain merchandise.
If I am comfortable with the asset liquidation required with the $1500 premium, I would select Case 3, the adjustable rate with $1500 premium, which provides a future credit line that is useable for any purpose, including the replenishment of financial assets.
Depending on which level of Premium Membership members select, patients can also access health care advocates for help with navigating complex issues, such as understanding insurance (policies, payments, treatment coverage, etc).
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