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Fourteen-year-olds take this rite of premature passage very seriously.
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Although detailed analyses of their phenotypes are underway, we found that Zfp277−/− MEFs from E12.5 embryos undergo premature senescence at early passages (Figure 1D).
These data suggest that a loss of centrosomal integrity, caused by a reduction in NEDD1, can lead to centrosomal abnormalities and premature senescence in early-passage MEFs.
To investigate whether this reduction in NEDD1 could cause premature entry of early-passage MEFs into senescence, cells depleted of NEDD1 were assessed for SA- β-gal activity on various days after NEDD1 knockdown.
Premature leakage of electrons allows their passage directly to oxygen, rather than being passed to oxygen to form water at complex IV, producing superoxide.
Many premature infants receive medications prior to the passage of meconium, which may be delayed until the ninth day of life [ 28].
Mutant lamin A (progerin) progressively accumulates in the nucleus with cellular age, resulting in premature cessation of growth in the later passages of HGPS cells (Goldman et al 2004).
In order to avoid premature uptake or degradation of NP during their passage through the small intestine, it appeared necessary to devise a form of local delivery system for NP.
Viable genetic variants with different sizes of NS1 gene products due to premature stop codons have been reported on laboratory passage of pathogenic avian H7N3 IAV but these mutations attenuated growth in chickens.
Forced expression of Bmi1 did not abrogate the premature senescence of Zfp277−/− MEFs at early passages (Figure 5A).
All experiments were performed between passage P5 and P15 after isolation to avoid premature replicative senescence.
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