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Martin Richards, a geneticist at the University of Huddersfield in the United Kingdom, says that the data look "persuasive and exciting," but should be considered only preliminary because the "sample sets are tiny at the moment" and small sample sizes can produce "misleading" results.
A preliminary sample size for each community was set based on the need to statistically represent the community household population at a confidence level of 95% and a confidence interval of 6.5%.
The accuracy of assembly was validated by PCR and Sanger sequencing for a set of randomly selected genes assembled and annotated from the preliminary sample (Additional file 2: Appendix S2, Additional file 3: Figure S6).
Based upon the reduction in explained variance and the need to keep the preliminary sample small, the first eight component vectors were selected to represent the data sets in the preliminary sample.
Table 6 lists the summary statistics for the preliminary sample.
The graph below shows the average results from the preliminary sample.
The result is his "Carpet Sample Set".
Inspired by the functional similarity between MAMDC1 and ITGAM, we performed a preliminary analysis of their potential interaction, by using genotyping data available (unpublished and refs [13] and [49]) for MAMDC1 SNPs rs961616 and rs2297926 (in the entire sample set), and for ITGAM SNPs rs11150614 and rs11574637 (respectively in the UK sample, and in the Finnish and Swedish sample sets).
The sample set of indigenous South Africans is relatively small, and because of this, the information about allele frequency and copy number variation is presented here as preliminary data.
Preliminary sample description: N=352 individuals: PCIW n=173 /CAU n=179.
Of note, no preliminary sample processing is necessary.
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