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The background was preliminary removed from the individual Cad and Tll patterns as described [ 18].
The trypsin solution containing detached cells was combined with preliminary removed incubation media and PBS and centrifuged at 1200 g for 20 min. Supernatant was removed, pellets were suspended in PBS, and 10% SDS was added to a final concentration of 1%.
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The work on Wednesday was preliminary, removing debris, highway signs and guardrails to make working space.
Preliminary analysis, removing respective primer lengths for each microsatellite size range, showed no relationship of primer length with microsatellite size or with levels of heterozygosity.
After examining the factor loadings on a preliminary analysis, we removed two items due to low communality (<0.40) and one item due to high cross-loading (>0.40) on two factors.
Sequences that caused an unacceptable loss of putatively informative sites (due to the sequence nonalignment or not masking well) or that formed long branches in preliminary analysis were removed.
After preliminary investigations with specimens removed from the spokes for the adjustment of the treatment parameters, fatigue tests, measurement of residual stresses and metallographic analyses of untreated and treated spokes were performed.
A preliminary processing of data removed non-expressed spots and those corresponding to buffer and operon controls.
Because Turkey Vulture (Cathartes aura) generated a long branch, it was removed after preliminary analysis (see Gibb et al. 2007; Slack et al. 2007).
First, the loosely adherent cells were removed by preliminary shake off (for 2 h at 225 rpm) and subsequent medium replacement.
Duplicate publications, protocol descriptions, abstracts, letters-to-the-editor, and incomplete or preliminary reports were also removed during the screening process.
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