For example, analyses of BAC end sequences and preliminary generation of BAC contigs by using the above libraries have been conducted.
In the second preliminary generation, pairs of virgin females and males from different field collections were mated, thus promoting outbreeding.
In the first preliminary generation, parasitoid cocoons were individually isolated in order to obtain virgin females and males, thus excluding multiply inseminated females and sperm-depleted males.
To obtain full siblings of C. glomerata for the initiation of experimental populations, two controlled preliminary generations were bred.
The Stroke-PROM was developed in four phases: (1) conceptual framework construction and preliminary item generation; (2) formation of the initial scale by the first item-selection process; (3) formation of the final scale by an item-revaluation process based on the second item-selection process; and (4) validation of the Stroke-PROM.
Strategies for the generation, preliminary design and structural optimization are proposed for the different patterns; in particular the optimization process is treated with mono-objective genetic algorithms, by minimizing the structural weight and imposing a constraint condition on the lateral stiffness of the building.
However, whereas rats did not seem to have any problems coping with blue-green algae enriched diet over a whole generation, preliminary studies in our laboratory indicated that the offspring of mice exclusively fed with blue-green algae diet had severe developmental problems.
Even though the results from only one cell is described here, currently, we are generating stable cells expressing these probes in selected NCI cancer cell line panels to be used as next-generation preliminary cancer drug-screening platform in high-throughput mode.
The thematic analysis will comprise of a six step approach including; (i) familiarization of the data achieved by reading each transcript, (ii) generation of preliminary codes, (iii) searching for themes from the preliminary codes, (iv) creation of thematic map, (v) specific definition and naming of themes and (vi) final analysis [ 14].
Thematic analysis consisted of a six step approach including; (i) familiarization of the data, (ii) generation of preliminary codes, (iii) searching for themes from the preliminary codes, (iv) creation of a thematic map, (v) specific defining of themes, and (vi) final analysis [ 24].
Since the Msh3 gene is on murine chromosome 13, triple heterozygous Msh2+/−Msh6+/−Msh3+/− were generated by breeding cis Msh2+/−Msh6+/− with Msh3+/− mice as a preliminary step to the generation of triple knockout animals.
