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The Agilent 2100 Bioanalyzer DNA 1000 chip was used to confirm the libraries' fragment length and obtain a preliminary concentration of the stock aliquot.
MDA-MB-231 were used for determining well occupancy in preliminary concentration curve experiments.
THP-1 (ATCC® TIB-202™) cells were cultured per ATCC instructions and used for determining well occupancy in preliminary concentration curve experiments as well as the three-color fluorescence data.
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Preliminary concentration-response analysis (100 µmM3 mM acetylcholine) within individual neurons suggested that 1 mM acetylcholine elicited near-maximal responses in both males and females, which could be reproduced following a five-minute washout period.
The concentrations of AEDs used in drug combination experiments were based on our preliminary concentration-response studies in adult zebrafish.
Stock solutions were diluted in LSM broth (90% IST plus 10% MRS) to obtain solutions with preliminary concentrations in the range of 0.25 128 μg mL−1.
We therefore designed a prospective observational study to analyze the variability of linezolid serum concentrations in relation to preliminary target concentration ranges in a heterogeneous group of critically ill patients with suspected infections.
These solutions were split into three aliquots: (1) 0.5 ml for preliminary Zn concentration measurements, (2) 1 ml for anion exchange chromatography to purify Zn for isotopic analysis, and (3) 1 ml for archive.
Interestingly, at the preliminary screening concentration (50 μM), all the triazolyl anaolgs displayed selective cytotoxicity against lung (NCI-H322 and A549) cancer cell lines in a dose dependent manner.
Preliminary RBP4 concentrations were determined by ELISA.
In preliminary experiments, concentrations of primers and probes were optimized to give low-threshold values (reporter fluorescence significantly above baseline fluorescence) and high PCR efficiency.
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