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Preliminary assay of the abilities of these compounds to inhibit the growth of TB5 Saccharomyces cerevisiae carrying the DHFR genes from M. tuberculosis, human and yeast indicated that 5-phenyl-6- 3R,4S -3,4,5-trihydroxypentyl pyrimidine-2,4-diamine selectively inhibited M. tuberculosis DHFR and had little effect on the human or yeast enzymes.
A preliminary assay of CXMDJ cells showed that three sequences, Ex8G, Ex8I, and Ex8K, were effective.
A preliminary assay of five rpl5 mutant strains using the near- (AGC) and non- (UCU) cognate reporters indicated that the K27E mutant of L5 tended to be generally hyperaccurate (data not shown).
A preliminary assay of 26 IDC samples (arbitrary 10 μl of DNA extract) by GP5+/GP6+ PCR demonstrated faint bands upon electrophoresis for five (19%) samples.
Therefore, to predict the effects of the most promising phenolic compounds (gallic and caffeic acid) on animal cells, a preliminary assay of cytotoxicity was made.
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After a preliminary assay on one of the MDR bacteria, P. aeruginosa PA124 (See supporting information Additional file 1: Table S2), the two best extracts were those from P. nigrum and T. occidentalis.
A series of different concentrations of ABA (1 500 µM) were tested in preliminary assays of stomatal conductance over time.
In preliminary assays of Drosophila larval behavior in dense populations, we noticed that larvae seemed to be attracted to regions of assay plates that had been occupied previously by other larvae.
For preliminary assays of enriched acrB libraries, the pre-culture was prepared by transformation of the mixture of plasmids into electrocompetent JA300A cells and growing the cells overnight in 10 mL LBA medium after 1 h of outgrowth.
Finally, in vivo use of quercetin as medicine suffers from the lack of approved formulation despite a first preliminary assay as adjunct treatment of prostatitis/chronic pelvic pain in humans [ 44].
A preliminary assay using phenolic extracts of Portuguese medicinal plants was made, in order to identify phenolic compounds from those extracts.
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