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Reads were preliminarily assembled using ABySS-P [ 96, 97] and SSAKE [ 98], and the results of these assemblies merged using PCAP [ 99] to create the final assembly.
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The generated sequences were assembled and preliminarily analyzed at the LGE Laboratoryy of Genomic and Expression at UNICAMP, Campinas, São Paulo, Brazil).
Thus, we preliminarily tested the capability of recombinant isomin to assemble in vitro into filaments, using the standard re-assembly conditions described for other IF proteins [ 24].
Assembling the molecular systems from preliminarily prepared metal ion and individual ligand fragments and analyzing the components of the total bonding energy (Pauli repulsion, electrostatic interactions and orbital part), we noticed interesting correlations between the orbital part and the simple formulas of the qualitatively defined ligand field stabilization energy (LFSE).
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