Sentence examples for preincubation for from inspiring English sources

However, preincubation for 10 min with Orai pharmacological inhibitors, YM58483 or Synta66, reduced the rate of Ca2+ influx observed in response to a second challenge with TNFα, to a value similar to basal (Fig. 2A middle and right).

In in vitro iso-treated hypertrophied cells, stimulation of Ca2+ influx by TNFα was unaffected by the preincubation for 1 hour either with control IGg2A or anti-TNFR1-antibodies (Ab) but was impaired in the presence of neutralizing TNFR2-Ab (Fig. 3A).

The neuroprotection was concentration and time dependent; preincubation for at least 24 h with a minimum of 10 ng/mL of PDGF-BB was required for maximal neuroprotective effect.

After preincubation for 30 min, the medium was replaced with KRB with the indicated concentrations of glucose or KCl (50 mM, equimolarly replacing NaCl).

In the preadsorption experiments, antibody binding was blocked by preincubation for 5 h at 4°C with a 20-fold molar excess of the peptide.

After preincubation for 3 min at 37°C, the reaction was started by addition of 170 µM PA substrate and incubated at 37°C.

The cycling conditions were 95°C for 5 min (preincubation), 95°C for 5 sec, 60°C for 15 sec, 72°C for 15 sec (amplification); 95°C for 5 sec, 65°C for 1 min (melting curve); 55 cycles were performed.

PCR was performed in duplicates in 96-well optical plates on Light Cycler 480 (Roche) under the following conditions: 95°C for 5 min (preincubation), 95°C for 5 sec, 60°C for 15 sec, 72°C for 15 sec (amplification), 95°C for 5 sec, 65°C for 1 min (melting curve).

The CYP preincubations for 0 min were used as the control incubations (100%), and error bars indicate the SD values.

The PCR cycle conditions were set as follow: a preincubation step for 10 minutes at 95°C followed by 40 cycles for tissue cDNA and 53 cycles for saliva cell cDNA; each cycle included 15 seconds at 95°C, 15 seconds at 60°C, and 15 seconds at 72°C.

Using four cephalosporins antibiotics (cefazolin, cefuroxime, ceftriaxone and cefepime), multiple regression analyses (MRA) were performed by varying the components of the MHA culture medium, proportion of the inoculum and preincubation time for different antibiotic concentrations.