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Data manipulation was preformed using a set of customised executable programs written in Visual Basics by José M.C.
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Three replicates were preformed using a different lot of McrBC.
BLASTN was preformed using a threshold of 0.001 for the E-value expectancy parameter, and setting the other parameters to default values.
K-means clustering was preformed using a Euclidian distance function with 1,000 iterations utilizing different values of K.
Quantification of KT intensities was preformed using a method adapted from that described earlier (Elowe et al, 2007).
Nucleotide sequence analysis of the mdh locus was preformed using a primer pair designed from VP0325.
25 Entry points were preformed using a 25-gauge needle (0.5 mm×16 mm).
Use a set of heel strips.
Liposomes were preformed using polycarbonate filters with a pore size of 400 nm and destabilized by addition of octyl glucoside to a final concentration of 1%.
The inelastic time history analysis is preformed using the direct integration technique considering a time step of 0.005 s.
Ab initio calculations have been preformed using Unrestricted Hartree Fock (UHF) method with 6-31G∗+ baset set.
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