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Due to their natural tendency to avoid bright environments, mice showed significant initial preference to the dark chamber (preferred chamber) and spent significantly more time (about 70% of the total time) there when allowed to move freely prior to conditioning trials (Figure 4).
This reversal of preference was reflected in a large negative value of the preference score, calculated as the percentage of time spent in the initially preferred chamber minus the percentage of time spent in the initially non-preferred chamber during the STM test (or other phase of the experiment).
Nicotine injections were paired with the non-preferred chamber and saline injections paired with the preferred chamber.
Since animals preferred the dark chamber, nicotine injections were always associated with the non-preferred chamber and saline with the preferred chamber.
During the days 2 and 4, each animal was placed inside its preferred chamber.
Only data from animals for which we could determine a preferred chamber during day 1 were included in the behavioural analyses.
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Time spent in the previously non-preferred and preferred chambers were recorded and the percentages of time spent in the non-preferred chamber were calculated and compared within and between the groups.
After nicotine conditioning, all three control groups exhibited a strong preference toward the previously non-preferred chamber by spending a significantly increased amount of time in there (Figure 4, p<0.01).
The proportions of time spent by each mouse in the non-preferred chamber were calculated and used as the index for conditioned place preference.
A three chambered CPP apparatus was used and nicotine was paired with the initially non-preferred chamber.
Following conditioning, the paired group spent significantly more time in the initially non-preferred chamber relative to saline-treated controls, suggesting CPP.
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CEO of Professional Science Editing for Scientists @ prosciediting.com