To filter for those transcripts most specific to hNCC that might reside in postnatal tissues, we performed tissue preferential expression (TPE) analysis (39), using SAGE data from 14 other normal postnatal human cells or tissues, initially excluding hES cells.
Additional file 12, Table S6, lists 349 2ROs preferentially expressed in brain (preferential expression measure >0.4).
Furthermore, allelic expression analysis showed preferential expression of the unmethylated paternal allele at the proximal promoter regions, which is consistent with an inverse correlation between methylation and expression.
Pharmacological analysis indicated preferential expression of mGluR5, while only a minority of the 3,5-DHPG-induced [Ca2+]i elevations were sensitive to an mGluR1 antagonist.
Interestingly, immunohistochemical analysis revealed a preferential expression of Prx I and II in Leydig and Sertoli cells, respectively.
Flow cytometry analysis supported this preferential expression of TNFRSF9 in CD8+ T cells at the protein level.
We used quantitative RT-PCR (qPCR) to show upregulation of FAS II genes in liver stages because our previous microarray analysis had indicated preferential expression in liver stages of P. yoelii (Tarun et al., 2008).
While sulfate was an ingredient of the HMB medium used in both benzoate and citrate cultures, comparative proteomic analysis indicated a significant preferential expression (over 11-fold) of arylsulfatase (Pput_2406) in benzoate-grown cells.
RT-PCR was used to verify preferential expression of genes identified in the TPE analysis.
Using significant microarray analysis (SAM) in the TIGR multi experiment viewer (MEV) toolkit, we identified 104 genes with preferential expression in indica (indica eQTLs) and 490 genes preferentially expressed in japonica (japonica eQTLs).
