These observations are not unexpected given the known preference of target predictors for guanine and cytosine bases and that long sequences have a higher probability of containing target sites.
In that case, although tendency of L1 to be inserted into genes related to diseases is not rejected, the statistical significance may suggest different sequence preference of target site selection by L1.
To examine a possible interrelation between the annotated biological function and the preference of target predictions for long and GC-rich sequences, the 1,000 longest and GC-richest, as well as the 1,000 shortest and GC-poorest transcripts were tested for over-represented and under-represented terms with the same significance cutoff.
Binomial analyses revealed that nonbonded birds did not have a significant preference for targeting dyads of a particular bonding class, whereas loosely bonded, strongly bonded, and pair-bonded ravens preferentially intervened in interactions of loosely bonded birds (see also Supplemental Experimental Procedures and Figure S2).
Importantly, the preference of DSB targeting annotated transcripts seems to be related to active transcription and therefore supports the model that gene expression in meiotic tissues play a role albeit clearly not the only one influencing the landscapes and magnitude of recombination in a particular genomic region.
The S. pombe retrotransposon Tf1 has been shown to exhibit preference for targeting the promoters of RNA polymerase II transcribed genes [ 56].
Despite the different mechanisms of dosage compensation, the preference for targeting active genes seems to be shared by all three species.
We also examined whether individual microRNAs had a preference for targeting genes in any of the three classes; however, a chi-square test at the significance level of 0.05 did not detect any such case.
Overall, the cost-effectiveness of COB was high as it produced widely adapted genotypes from a small breeding programme, and knowledge of farmers' preferences increased the efficiency of targeting new germplasm.
Corridors depend on both the composition and spatial arrangement of the landscape, and the movement abilities and landscape preferences of target organisms.
