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We want to see less of you, or preferably none at all.
Those of us who suffer the noise and air pollution generated by Heathrow want fewer not more aircraft screaming over our homes, and preferably none.
The bisulfite sequencing primers were designed to amplify regions with preferably none and maximum 1 CpG site in the forward and reverse primers and to flank the area amplified by the qMSP assay.
For example, it is recommended that no more than 20%, and preferably none, of the compartments should have less than five expected gold particles (Mayhew and Desoye 2004; Mayhew et al. 2004) This may influence the choice of compartments or the numbers of observed gold particles to be counted.
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None of this can be accomplished without a hero, preferably one who verges on the herculean.
In the Spanish viceroyalties of Aragon, Catalonia, Valencia, and Navarre and in those of Mexico and Peru, none but Spaniards, preferably Castilians, were ever thought of at all, with the exception of one or two Italians.
The general feeling is that it is better to have some independence than none at all – and preferably, total independence with transparent, non-political governance structures.
Use odd phrases and words, preferably old ones, like "It is none of your concern.
None of whom have anywhere to go but down, preferably off a high adobe wall, in a hail of bullets.
They piled their own falsehoods on top of the Clintons' evasions, none more preposterous than the notion that Mr. Foster was murdered, preferably by Hillary Clinton herself.
Preferably now.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com