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SMF-1 may thus be the counterpart of NRAMP1 in the worm, being predominantly required for Fe uptake.
This observation is consistent with a previous study of Gram-positive Lactococcus spp. lipoprotein processing that found that lipid modification is predominantly required to retain proteins at the membrane cell-wall interface [36].
It was surprising to find that hsf-1 was predominantly required during development and again, to a lesser extent, during adulthood.
For both L1 and IAP the three mechanisms are in place within spermatogonia, however the derepression of IAP in MiliKO spermatogonia indicates that DNA methylation and or the piRNA pathway are predominantly required for the maintenance of IAP silencing therein.
Finally, it seems that Gsc and the Vent1/2 genes are predominantly required in their normal side of expression, while after removal of all three they seem to be dispensable for embryonic pattern formation.
These phenotypes are equivalently rescued by low level, neuron-specific expression of Drosophila or human wild-type spastin transgenes, indicating that Spastin is predominantly required in neurons, and functions similarly in both organisms (Sherwood et al., 2004; Du et al., 2010).
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This task predominantly requires behavioural inhibition of premature responses that have negative consequences.
However, regardless of the miRNA biogenesis diversity, the stability and silencing activity of mature miRNA predominantly require AGO effector proteins [ 7– 9].
The populations they serve predominantly require residential care because of progressive chronic disease and cognitive impairment, resulting in disability and loss of function, and not just for frailty alone [ 5- 7].
From our results it is plausible that during the pre-symbiotic stage, transcriptional activation by Nod-LCOs predominantly requires NSP1, but at the same time, yet unidentified regulators, e.g. GRAS TFs, might still come into play.
By exploring the effect of MEK/ERK and PI3K inhibition on these RTK-regulated gene expression profiles, we find PDGF-mediated transcription displays greater PI3K dependence, while FGF-mediated gene expression programs predominantly require ERK activity.
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