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Systematic mutagenesis studies whereby each of the four proline-rich motifs was assigned function and SH3 domain specificity showed that motifs 1, 2, and 4 (from the N-terminus to the C-terminus) predominantly bound Crk, whereas motif 3 was more promiscuous and bound SH3 domains of Nck, Ponson/CAP, and ArgB2 [ 159].
Despite these differences the dissolved fraction (fd) and partitioning coefficient (Kd) distributions are similar, illustrating that P is predominantly bound to PM fractions.
An analysis of the eluates revealed that Fe is predominantly bound in insoluble degradation products, whereas a considerable amount of Mn is in solution.
By using Western blotting and a dot blot assay in combination with size exclusion chromatography, it could be concluded that selected Affibody ligands predominantly bound a non-aggregated form of analyzed Aβ peptide, which we speculate to be dimeric Aβ.
Whereas Sb was mainly bound to the residual fraction and partly also in the reducible fraction (Fe-oxides), As was predominantly bound to Fe-oxides (up to 71% of the total As content).
Co-labeling with HKI-CFP and MitoTracker-YFP (Fig. S1) supports the interpretation that HKI, even when overexpressed, is predominantly bound to mitochondria.
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Human seasonal viruses almost exclusively attach to N-acetylneuraminic (sialic) acid receptors at α-2,6 linkage, whereas avian influenza viruses predominantly bind to α-2,3 sialic acid linkage.
We found that OSK predominantly bind active somatic enhancers early in reprogramming and immediately initiate their inactivation genome-wide by inducing the redistribution of somatic TFs away from somatic enhancers to sites elsewhere engaged by OSK, recruiting Hdac1, and repressing the somatic TF Fra1.
Even if the hypothesis has to be examined in more detail, we conclude that TcdA and TcdA1 1874 predominantly bind to different but not independent receptor structures.
Since soluble FcγRI was not available to us, we also investigated human monocytes that predominantly bind IgG through this receptor [14].
Based on re-analysis of previously published global binding studies performed in mouse ES cells, we found that Ring1b predominantly binds and represses genes with CpG-'rich' promoters.
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