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However, MDAMB231CXCR4+ tumor tissue slides showed somewhat different staining profile, with cytoplasmic and membrane predominant staining.
Immunostaining of sections of HFK (14 20 week) demonstrated predominant staining in the nephrogenic zone and renal stroma, while mature tubules were devoid of staining.
Successful harvesting of cells from new onset diabetic mice with less than 1% dead cells, predominant staining with death probes and low specific staining of apoptosis imply that few apoptotic cells are found in lymphoid organs in situ.
Strong β-catenin expression was noticed in hyperplastic ATII cells, in particular in areas of bronchiolization (Figure 4b), as assessed by immunohistochemistry. Gsk-3β showed a very similar expression pattern to β-catenin, with predominant staining in bronchial epithelial and ATII cells, as well as endothelial cells in donor lung tissues (Figure 5a, arrowhead).
In fact, virtually all-previous studies using these and other commercially available antibodies have reported predominant staining of cell nuclei with occasional cytoplasmic staining in human fixed-embedded tissues [7], [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18], [19], [20], [23].
The predominant staining pattern for CRPC specimens was strong and diffuse.
Similar(36)
In HER2-positive tumor samples containing low expression levels of CAV1, HER2 exhibits predominant membrane staining.
For ITGA6, membranous staining of myoepithelial cells was predominant while staining of luminal cells was seen less frequently.
Since the higher molecular weight multimers of EAAT1 and EAAT2 were not consistently detected, we analyzed the predominant strongly staining monomers of EAATs on Western blots.
Although in multivariate analyses ADA3 expression was not an independent marker of survival, predominant nuclear ADA3 staining in breast cancer tissues correlates with ER+ expression and together serves as a marker of good prognosis, whereas predominant cytoplasmic ADA3 expression correlates with ErbB2+/EGFR+ expression and together is a marker of poor prognosis.
Predominant nuclear ADA3 staining correlated with ER+ status.
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