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For cases showing mixed intensity, the predominant intensity was selected as the final IHC score.
If there were areas of a variety of staining intensities, the predominant intensity was chosen.
Scores were given for the predominant intensity of staining, on a scale of 0 3 (0=negative, 1=weak, 2=moderate and 3=strong).
Nuclear intensity was denoted as no, weak, moderate or strong, with corresponding scores from 0 to 3, referring to the predominant intensity.
This difference can vary from a subtle decrease in intensity under the printed spot to a much more predominant intensity variation that seriously affects the accuracy of the data.
The grade for each cell type was the product of average labeling intensity and the relative percentage of cells (0%, 0; up to 1%, 1; 2–5%, 2; 6–25%, 3; 26–50%, 4; more than 50%, 5) labeled at the predominant intensity.
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In this regard, it must be kept in mind that, except in 100 km races, the predominant exercise intensity of most events studied in this report corresponded to zones 2 3 (Figure 3).
AFI images were diagnosed based on the predominant color intensity.
In cases with heterogeneity, the predominant staining intensity (> 80%) was counted: (0) negative, + (1) weak, ++ (2) moderate, +++ (3) strong.
In the abovementioned relationship between pre- and post-treatment VAS scores of predominant pain intensity on a 100 mm scale, scatter is involved in the S 2 -values.
Percentage of positive cells (0, 5, 10, 20, 30, …, 100%) was then multiplied by the predominant staining intensity score to get a value ("multiplication score") for each core, ranging from 0 to 300 (Fig. 1G; Glaessgen et al. [ 21].
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