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Predictions were mapped in a geographic information system environment (ArcGIS 9.0, ESRI, Redland, CA).
67 predictions were mapped to 41 unique P. knowlesi orthologs available on the array, almost all showed substantial upregulation (>5×) in the late schizont stage (Figure 4).
Subsequently, the disorder predictions were mapped onto the MSAs used for the phylogeny reconstructions and visualized as heatmaps following the same order as the sequences in the specific phylogeny for each protein (fig. 3A).
To identify the genes within the bovine genomic contig, sequences from human and bovine RefSeq, and TIGR bovine Gene Indices, along with the known bovine BPI-like gene sequences from GenBank and Ensembl bovine gene predictions, were mapped onto the contig using the GMAP and Exonerate programs.
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Following the ZOOPS model, we predict at most one motif occurrence per sequence in the cut dataset; the cut heuristic means that more than one occurrence per sequence may be predicted when these predictions are mapped back to the original dataset.
Probe coordinates and RNA structure predictions are mapped to UCSC [ 39] and RefSeq [ 40] gene tracks with at least 10% overlap of probes and predictions.
To produce the program output, the final set of FS predictions is mapped back to the initial metagenomic sequences and combined with gene predictions of MetaGeneMark, thus producing the full list of genes with or without frameshifts.
Target predictions from miRanda were mapped to genomic loci and filtered to include only those supported by at least one experimental dataset.
The Glimmer predictions for this supercontig were mapped back onto the original 454 contigs to avoid designing probes overlapping with parts of the inserted artificial sequences.
The 3,478 QRNA predictions analyzed by microarray were mapped to the mouse-human UCSC genomic alignments (mm6-hg17) and were subset to the same regions analyzed by Cheng et al. [ 9] (i.e. not repetitive regions, for example), which were determined from the probe positions used in the tiling analysis (coordinates were converted to the hg17 genome release using the UCSC LiftOver tool [ 17]).
Miranda predictions used Ensemble IDs, which were mapped to RefSeqs using UCSC genome table browser.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com