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As discussed below, a high number of false isozymes, triggering false dispensable predictions, were detected in this refinement step.
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The barrier effect, the magnitude of which matched the predictions, was detected during construction.
In total, 77% of the 1637 ortholog predictions are detected by GreenPhyl, 45% by Inparanoid and 35% by BBMH.
In the following two steps (see Figure 1), these predictions are aligned and annotated, such that redundant matches are clustered and homologous predictions are detected.
In this research, two putative uncharacterized genes without any gene function information, gene annotation, expression sequence tag(EST), transcriptome data or protein domain prediction were detected in response to heat shock.
If perfect prediction is detected, the perfectly predicting covariate and the perfectly predicted observations are dropped from the analysis.
The simplest is to abandon proper imputation if perfect prediction is detected — that is, to use the estimated parameter θ ˆ rather than a draw θ ∗ from the posterior.
For homology prediction, pseudo-genes were detected and dropped, gene models don't have synteny support but have higher quality score and less frame shift were retained.
Therefore, we analyzed the region upstream of each TSSs and for 16 of them clear σ70 or σ38 −10 recognition elements with high scores from two different prediction methods (see below) were detected at the right position upstream of some 5' ends described in this section (Table 2).
In addition to the deleterious predictions, protein regions under evolutionary constraint were detected using three evolutionary conservation-based indicators: GERP++ [ 22], phyloP [ 23], and SiPhy [ 24].
Positive impacts were detected, despite our prediction that a small MPA would not provide protection to highly mobile taxa.
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