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Twinscan and EuGene predictions were aligned to TIGR5 genome using BLAT.
Canine proteins inferred from the gene predictions were aligned against all canine transcripts with Exonerate using the coding2coding model.
The ZF exon protein sequence from all the human cluster 19.12 genes and their corresponding chimpanzee predictions were aligned using ClustalW (default parameters) [ 11].
By applying the general model described by Eq. (7) to all the major types of vertebrates (including endotherms: primates, dolphins, mammals and birds, and ectotherms: fishes, reptiles and amphibians), we found that the actual brain masses and model predictions were aligned well in a log-log plot.
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In the following two steps (see Figure 1), these predictions are aligned and annotated, such that redundant matches are clustered and homologous predictions are detected.
Each putative single-copy protein ortholog cluster recovered from orthology prediction was aligned using the T_Coffee software version 8.93 (Notredame et al. 2000).
Searches on the Joint Genome Institute (JGI) website were done against all gene prediction models, and gene predictions from different software were aligned to each other as well as to EST sequences when available, to select the best prediction.
To reduce false positives due to variation in gene prediction process, gene sequences were aligned against genome sequences of other isolates rather than directly comparing gene sequences.
For example, the 0.4 ml juice was the larger outcome of a gamble between 0.1 and 0.4 ml of juice, and hence the dopamine responses were aligned to the prediction error occurring after the solenoid duration that would have delivered 0.1 ml of juice (∼25 ms).
Of our predictions for the mouse genome, 68% were aligned to the genome of an organism at an evolutionary distance ranging from dog and cow to (any of the) fish and showed predicted secondary structure conservation with that organism (Figure 4).
For the GeneSeqer predictions, the longest representative CDS was selected if multiple transcripts were aligned to one locus.
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