Exact(2)
To avoid false positive predictions, we excluded signal peptides before running TMHMM2.0 and SOSUI.
To restrict ourselves to the most novel predictions, we excluded any predictions in which the gene had previously been annotated to any GO term in the same branch as the predicted GO term.
Similar(6)
Of all new predictions, we exclude HIV-related predictions, which would, due to their large number, make literature search difficult.
To more fairly compare the prediction methods, we exclude the 226 proteins from Set600 that share >90% sequence identity with the CMAPpro training set.
We excluded redundant predictions for the same genomic location and only kept the prediction with the highest score.
Finally, when looking for novel ncRNA genes (as opposed to regulatory elements that might be located within protein-coding genes), we excluded any predictions that overlapped with previously annotated genes (protein-coding or non-coding), pseudogenes or transposons in FlyBase release 5.4 [39].
To obtain high-efficacy targets, we excluded target predictions with a context score > −0.1 (27).
In general, TFs with very similar motifs (which we excluded from our prediction) can in reality jointly bind to the DNA sequence and regulate the transcription of the target gene.
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