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The performance of each model was evaluated by comparing the predictions of seed development times with nine independent recorded seed development times in the field.
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In addition to the in silico prediction of seed regions, we examined changes in individual tRF isoforms with age.
In addition, we used the clustering results presented in Table 1 to compare the prediction performance of SEED with the other methods.
The study of physiological, molecular and genetic factors involved in seed ageing and tolerance and predictions for seed shelf-life might benefit from the development of a method to study seed ageing also in dry seeds and at temperate temperatures.
In addition, we found that prediction as a function of seed mass and HCNSG was greater than prediction as a function of years since introduction for noxious classes, but not for naturalization or weeds.
The screen has discovered the rules underlying this seed toxicity and allows prediction of the 6mer seed toxicity for any siRNA, shRNA, miRNA with a known 6mer seed (https://6merdb.org).
A novel approach called TargetSpy seed (Sturm et al., 2010) restricts the set of seed matches to predictions containing a perfect 7mer.
DOI: http://dx.doi.org/10.7554/eLife.05557.008 10.7554/eLiFigure57.009 Figure 2 figure supplement 4. Germination kinetics of field-collected seed batches from 2012 and model prediction of temperature during seed maturation.
Bioinformatical prediction is based on the degree of seed region match with the 3'UTR of target genes.
To explore the influence of seed region composition in the prediction of observed changes upon miRNA modulation, Targetscan's non-conserved predictions were categorised by their length and composition of seed region.
Many miRNA target prediction software programs were developed using the features of seed sequences [ 14- 19].
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