This theory and its predictions have recently been extended to genes with caste-limited expression in social insects (Linksvayer and Wade 2009) allowing one to distinguish adaptations based on what H. Robinson has called 'we genes' from those founded on 'me genes' in terms of distinctive patterns of sequence polymorphism and divergence.
Whilst the concepts we present are applicable to all host and vector stages of the life-cycle, we focus on parasite traits expressed during the erythrocytic phase of infections because the necessary tools and techniques to experimentally test our predictions have recently become available.
To reveal the underlying intricate mechanism of information propagation, the problem of repost behavior prediction has recently drawn extensive attention.
In silico protein target prediction has recently become the subject of intense research [5 12], since it helps us both to infer and to understand molecular bioactivities.
This prediction has recently been confirmed by a study showing no change in quiescent episodes, but an increase in the contraction frequency and duration of episodes of propulsive contractions after short-term exposure to cholera toxin [30].
Energy-based prediction has recently been extended to include a penalty term that reflects the free-energy cost associated with disruption of pre-existing secondary structure at potential target sites on the mRNA [20] [22]; such a term could, if desired, be easily incorporated into our approach.
Currently reliable clinical or molecular markers to evaluate response are still lacking, even though metabolic response prediction has recently been evaluated as a method [ 25].
Moreover, since the accuracy of helix‐helix contact site prediction based on intra‐protein contact prediction has recently reached 70‐80% [ 67, 69, 70, 73], we propose to extend these previous studies.
This prediction has recently been confirmed; both M. luteus Rpf and B. subtilis YocH have murein hydrolase activity in zymograms (Mukamolova et al., ms. in preparation; Ravagnani et al., ms. in preparation).
Specifically, this prediction has recently been tested over all of the years of the long-term badger study in Wytham Woods [ 19], in which TS and GS were consistently uncorrelated without exception (using two different methods of territory size estimation), leaving open the question of what independently limits these variables.
