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To validate these predictions, expression of csRNAs in Streptococcus mitis, Streptococcus oralis, Streptococcus sanguinis, and from a Streptococcus thermophilus plasmid was tested by northern blot analysis.
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In line with our prediction, expression of DEK has been shown to be targeted and activated directly by E2F [ 29].
Recently, Zhang et al. [ 92] proposed a method to infer MRMs by integrating miRNA-target predictions, expression profiles of miRNA and mRNA and the topological structures of protein protein interactions (PPIs).
Consistent with this prediction, conditional expression of dilp6 in head and abdominal fat body extended female lifespan (Table 1).
In accordance with this prediction, transient expression of MdBV PTP-H2 or PTP-H3 in Drosophila S2 cells led to a reduction of phagocytosis of E. coli by these cells [ 32].
To validate this in silico TPE prediction analysis, expression of seven putative epithelial-specific genes by PBEC and KC was assessed by reverse transcriptase polymerase chain reaction (RT-PCR) in nine different cell types.
On the other hand, the identification of these tissue-preferred URSs and their motifs will benefit not only the designing of synthetic URSs but also the computer prediction of expression patterns of genes in other closely related species.
This information can also be used for other objectives such as the discovery of sequences important in X-chromosome inactivation, and the subsequent prediction of expression status of individual genes [ 24].
As an initial test of these predictions, the relative expression of ICP0−GFP or ICP0+GFP fluorescent reporter proteins was compared in cells inoculated with 2.5 pfu per cell of HSV-1 0−GFP (ICP0−) versus HSV-1 0+GFP (ICP0+).
We review computational predictions of expression from the promoter architecture – the set of transcription factors that can bind the proximal promoter.
Since some protein sequences are 5′ truncated or predicted starting from an upstream point, we manually checked the prediction of top expression of transcripts containing signal peptides.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com