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We also evaluated the performance of top ranked predictions by determining whether known target proteins appeared in the top 10 or top 50 high-scoring predictions.
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We tested the second prediction by determining whether texture signals "followed" single neurons as stable attributes.
Because strong stimulation of mGluRs with bath-applied DHPG has persistent effects on eIPSCs and DSI [14], [20] that could confound interpretation, we tested this prediction by determining if repetitive elicitation of DSI would affect eCBmGluR.
In Material and Methods, we formalize this prediction by determining the posterior probability, the probability of a tree given the observed sequence data.
We experimentally tested this prediction by determining the percentage of engulfed surface area for each particle for different time points after initiation of phagocytosis, and comparing this result with our simulations, in which simulation time was matched to actual time.
We verified this prediction by determining the structure of a fusion protein with the first nine ANK repeats of AnkB fused at the C-terminus of Nav1.2_ABD-C at 2.5 Å resolution.
The validity of cluster predictions was probed by determining if some the genes in each cluster encode protein with similar annotated functions (PlasmoDB.org).
Additionally, this information may allow more accurate prognostic predictions, for example, by determining the expression of molecular markers of prognosis and metastasis (e.g., HOTAIR).
Having determined the posterior distribution of model predictions, there is now a direct link between different predictions and parameters, which can be exploited by determining how predictions relate to each other and to the model parameters.
We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter.
The most reliable predictions can be achieved by determining the level of expression of MUC1.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com