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The second approach to exploit conservation data is to use it initially to make contact predictions and subsequently use the predictions for quality assessment.
For the z-score statistics we referred to a standard statistical method for the annotation of TF hits (Rahmann et al., 2003), which balances the expected number of true and false positive binding site predictions and subsequently applied a z-score statistics [as used for instance by oPOSSUM (Ho Sui et al., 2007)] to detect any binding site enrichment in a given tissue category.
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Most importantly, removing incomplete transcripts and underrepresented variants increases our confidence in the transcripts remaining for orthology prediction and subsequently, the reliability of the inferred relative expression of each predicted ortholog.
Two different ANFIS networks (by changing the training optimization algorithms) were compared with evaluation of networks accuracy in bubble point pressure prediction and subsequently the suitable network was determined.
In addition, the read-lengths generated during sequencing influence sequence assembly, gene prediction and subsequently the functional analysis.
We propose a joint strategy that combines fragmentation trees and multiple kernel learning (MKL) to improve molecular fingerprint prediction and, subsequently, the metabolite identification.
The phase IIB study results were in accordance with the predictions made and subsequently used to develop an exposure response model to analyze PASI-75 and PASI-90 response rates.
The same small data set being used for constructing the prediction model and subsequently evaluating the prediction performance tends to give over-optimistic estimates.
The simulation involved prediction of swaging force, which was subsequently compared with force measured experimentally using own designed force detection system, although other parameters, such as strain, strain rate, stress and temperature were also predicted and subsequently compared to experimental data.
We included studies that both developed a prediction model and subsequently evaluated it on separate data, as well as studies that only described the evaluation (validation) of one or more existing prediction models in other participant data.
The recent systematic reviews highlighted the need for an independent study to identify the existing prediction models and subsequently validate and compare their performance to support the current recommendations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com