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First, the predictions allow us to ascertain how well the model generalizes.
It is important to note that the predictions allow us to plan number and type of dental personnel required, not which child needs therapy [ 44].
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To also look at more divergent repeat proteins we chose three proteins that were predicted to be ARM in recent years [21], [22], [23] and where multiple sequence alignments and consensus secondary structure predictions allowed us to confidently predict domain boundaries.
However, since including all sources of predictions allowed us to perform the comparison using a larger set of both 'unknown' and non-'unknown' literature-based annotations (data not shown), we included all sources in our further analyses.
The qualitative distinction between these predictions allows us to detect a signal of gene flow.
First, high-confidence miRNA target predictions allowed us to characterize the overall functional spectrum of miRNA-regulated protein complexes.
Data mining of genomic databases and bioinformatic predictions allowed us to determine that plants also possess lipocalins, which were classified as temperature-induced lipocalins (TILs) and chloroplastic lipocalins (CHLs) [ 4].
Similarity and secondary structure predictions allowed us to determine that these five families share an RNase H fold and to identify, unambiguously, the catalytic triad as well as the IR DNA-binding regions.
When we compared untreated samples with cells that had been treated with vitamin D3 for 14 days, we found that a small number of region types differed significantly from the linear model prediction, allowing us to derive a lineage plot for HL60 differentiation.
This classification identified eight predictions which allow us to discuss the existence of active regulations.
Extrapolating from the sample set, we would estimate that application of this review process to our total set of 608 manual 'unknown' annotations that have corresponding InterPro predictions would allow us to update 145 of the annotations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com