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This observation is not surprising; while MD does not define a completely new type of disordered region, it averages scores from several prediction methods and other sequence properties to define a new, refined score predicting disorder.
We demonstrated that methods predicting disorder based on different concepts identified very different "flavors" of disorder.
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These modifications may be considered as molecular signatures for predicting disorders of development and for identifying disease states.
To get more insight into the evolution of disordered regions, we predicted disorder in the human proteome using MULTICOM-REFINE.
SLiM prediction was executed with both predicted disorder and relative local conservation masking ('Bothmask'), disorder masking alone ('Dismask') or neither ('Nomask'Nomask
For the 1,266 regions from SwissProt, the predicted disorder closely matched the corresponding predictions for the 75 with known structure.
Combining disorder prediction and conservation data, many regions of conserved predicted disorder were found within protein domains.
In order to experimentally confirm the disorder predictions, we have expressed, purified and characterized the large regions of predicted disorder of Henipavirus N and P proteins.
They developed a neural network model to predict disorder, trained on regions of missing electron density in X-ray structures and disordered regions in NMR structures.
Finally, we demonstrate how by combining results from secondary structure prediction, different disorder predictors and MD, one can estimate the type of the predicted disorder region (Fig 4C D).
The N-terminus and the C-tail, with high predicted disorder in all arrestins (Fig. 3C F), are biologically important.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com