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The dysregulated known miRNAs were predicted to target about 30,846 genes based on RNAHybrid prediction.
Figure 1 miR- 145, miR- 221 and miR- 494 are predicted to target human ATF6.
PremiR-223, a miRNA increased in the CF lung but not predicted to target ATF6, was included as a control.
A premiR for miR-223, a miRNA not predicted to target ATF6, did not decrease luciferase activity.
Figure 3 miR-221 is increased in trachea and bronchi and is predicted to target murine ATF6 3'UTR.
Interestingly, miR-135b is predicted to target a leucin zipper putative tumor suppressor 1 gene (LZTS1).
Consistent with these reports, Porphyra miRNAs are predicted to target coding regions.
There is one miR that is predicted to target SHIP, miR-155.
miR-144, which potentially targets Rb1 as indicated above, was also predicted to target PTEN.
miR-103 and miR-107 only differ by one nucleotide and are predicted to target the same genes.
Eight miRNAs had 222 predicted targets in common; for example, mir-29b was predicted to target 196 of these.
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