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BACs predicted to span the arms of chromosomes 1 and 2 were identified for use as FISH probes (Table S3).
In conformity with the secondary structure of proteins from Gram-negative bacteria, spirochetal inner membrane proteins are predicted to span the lipid bilayer in α-helical hydrophobic stretches approximately 20 amino acids in length.
Moreover, in CpsA the DNA-PPF domain was predicted to span the third putative membrane segment with the major portion of the domain located extracellularly.
PSEN2 is a transmembrane protein with 448 amino acids and a molecular weight of 55 Da. 17, 18 It is predicted to span the lipid bilayer nine times.
CD20 represents a non-glycosylated, 297-amino acid (33 37 kDa) phosphoprotein that is predicted to span the plasma membrane four times [ 30].
Three BAC clones from a DL genomic library were predicted to span all of pMUM003 (04D12: 110 kb, 051B: 99 kb, 048F: 98 kb) and these clones were selected for further analyses.
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The gene for PY_PFB0505c, encoding beta-ketoacyl-acyl carrier protein synthase III, is predicted to be spanned by two separate contigs (Genbank ID 23485908 and 83285534) that encode the amino and carboxyl sequences of the protein.
Full-length huntingtin is now thought to comprise a large α-helical solenoid (repeated units arranged in a continuous superhelix/coil) (12) rather than a globular protein, as its ∼3144 amino acid length was predicted to be entirely spanned by loosely conserved HEAT/HEAT-like repeats (13).
NATs are highly hydrophobic proteins and are predicted to possess membrane-spanning helices (Schwacke et al. 2003).
In summary, a substantial anaplerotic amino acid flux into the CAC is predicted to restore both spans of the CAC, maintaining [NAD+→NADH], oxidative phosphorylation, and energetic viability.
A cyclic pushover test of a three-story steel plate shear wall (SPSW) specimen was conducted to investigate the seismic behavior of this system when plastic hinge was predicted to develop along the span of horizontal boundary elements (HBEs).
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