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To test this hypothesis, we engineered a PhoQ PD mutant that contains an internal disulfide bond predicted to restrict conformational dynamics between α4 and α5 and the α/β-core.
They also contain an Mrr system that is predicted to restrict methylated DNA (usually HhaII or PstI methylated DNA) that is 100% conserved between both strains (Additional file 5A).
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Note that 17 of the peptides are predicted to be restricted by more than one HLA class I allele, resulting in a total of 175 unique peptides.
Proteins predicted to be restricted to Nematoda and novel in A. crassus were significantly enriched in signal peptide annotation compared to conserved proteins, proteins novel in Metazoa and novel in Spirurina (Fisher's exact test p <0.001 ; Figure 3).
In regions in which this process is well advanced a high proportion of species are thus predicted to have become restricted to protected areas.
As a result, diffusion is predicted to be relatively restricted when the fiber data are analyzed (12 14).
On this basis it would be permissible for parents and doctors to elect not to preserve the life of a newborn predicted to have a restricted life.
It is the first zinc transporter predicted to have a fetal restricted expression.
Furthermore At3g19890, the transcript ath-MIR2939 was most confidently predicted to target, appears to be restricted to male gametophyte development (see Additional file 1: Table S2), peaking in expression at the microspore stage.
Therefore, NK-cell inhibition is spatially restricted and is predicted to be efficient only if the engaged activating and inhibitory receptors are localised within relative proximity.
Consistent with a miRNA hypothesis for schizophrenia, restricting to genes predicted to be affected by DGCR8 haploinsufficiency (Stark et al. 2008; Merico et al. 2014) tended to increase estimated effect sizes (Table 2 and Figure S1), despite the decrease in number of variants per subject.
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