We show that this mutation results in the generation of two mutant transcripts which are each predicted to generate a truncated protein.
Thus, the T-DNA insertion in the wrky25-2 mutant results in generation of a truncated WRKY25 transcript that is predicted to generate a truncated WRKY25 protein lacking the C-terminal WRKY domain, which is important for DNA binding [ 37].
Sequence analysis of the TCAP gene revealed a novel homozygous non-sense c.244C>T (p.Gln82X) mutation in exon 2, predicted to generate a truncated protein of molecular mass consistent with the Western blot findings.
An alternative translation initiator is present in exon 7 and predicted to generate a protein of 1033 amino acids.
Mutations that are predicted to generate a truncated protein similar in size to GLI3 repressor are observed in humans with Pallister-Hall Syndrome (PHS) and renal dysplasia [8].
Consistent with this hypothesis, cyclic equibiaxial stretching at 0.01Hz, which is predicted to generate a negligible perturbation in fiber tension (cf. Fig. 2), did not cause a significant change in cell spreading area (Fig. 3C E and Video S2).
