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Unlike FNN, however, FNP does not possess the lic operon, which is predicted to attach choline residues to the LPS [20]; only the licC and licD genes, encoding phosphocholine cytidylyltransferase and a phosphotransferase, respectively, are present.
Two small α-helices predicted to form in the HRV-QPM VP1 and VP3 footprints may disrupt receptor binding but since ICAM-1 is predicted to attach at differing angles in different HRV strains [8], it may be suitably flexible to overcome such obstacles.
ATCC 334 is predicted to secrete 361 proteins, of which 48 contain lipoprotein signal peptide and are predicted to attach to the cytoplasmic membrane.
This indicates that the genes identified in these fungi encode a conserved asparagine residue that is predicted to attach to a glycan chain to facilitate various co- and post-translational modifications and enhance the stability, transport and secretion of proteins [ 61, 62].
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Based on the crystal structure of the complex, YpAzF would be predicted to covalently attach to the LapD dimer half-side that engages with LapG via its GWxQ loop, whereas VpAzF points towards the other protomer that uses the interaction surface distal to its GWxQ loop.
The H flame, which relates to the same region of the stability map as the L flame, is also predicted to be attached.
Pridmore et al., (2004) identified cell surface components (LJ0382, LJ0391, LJ1128, LJ1711, LJ1839) in the human isolate thought to be unique to NCC 533 and predicted to be secreted and attached to the cell surface.
Another 67 are predicted to be secreted or non-covalently attached to the surface.
The latter enzyme is predicted to participate in the conversion of the C1 metabolite attached to tetrahydrofolate.
Treatment with RNase A would be predicted to produce a VPg-pUp product because RNase cannot remove the final attached nucleotide from the labeled VPg.
While three of these are predicted to be secreted and one (EF0201) is probably cytoplasmic, the others are predicted to be attached to the bacterial surface through an anchor (five lipo-anchors and one LPxTG anchor) or cell wall binding domain (one, EF2860) or even as integral membrane protein (one, EF1264).
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