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However the presence of specific transcripts generated at predicted termination sites, suggests that RNAse activity on these transcripts is minimal.
Altogether, 684 3'UTRs with internal termination sites could be determined, whereas 511 3'UTRs end at predicted termination sites.
sT-Ag, for example, which in SV40 and other PyVs contributes to cell transformation (27, 29, 33 ), had a predicted termination site within the LT-Ag intron in only 2/7 RacPyVs analyzed.
The clusters rarely contain other genes than the csc family members, as based on the criteria of correct gene orientation, small intergenic distance and absence of predicted termination sequences.
Terminator T1 must be weak, as it precedes the highly transcribed polycistronic region that includes genes petB and petD, and indeed its predicted termination probability is low in our model.
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We cannot predict terminations of activated seismicity at the present (Mar.
The opposite result was observed for RNA isolated from rickettsiae grown in L929 cells where the majority of the RP827 transcripts protected a 187 base portion of the probe indicating termination at the predicted terminator site.
RT-PCR and qPCR were used to test whether the predicted terminators were related to transcription termination in M. hyopneumoniae.
The reverse primer contained the reverse complement of both the predicted translation termination codon and the preceding 20 25 nucleotides at the end of the ORF (Supporting Information Table S1).
Results for 62 male DMD subjects are provided in Table 1, including data relating to the identified mutation, the exons affected by the mutation, the location of a predicted premature termination codon, the isoform(s) affected as inferred from mutation location, and results of the neuropsychological assessments.
SBMO2-injected embryos produced reduced levels of wild-type hadp1 transcript, along with an alternative transcript (supplementary material Fig. S6A,B) that lacked exon 5 and encoded a predicted premature termination product (data not shown).
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