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For this reason, sizeable portions of the other predicted sequences were expected to yield many useful gRNA predictions.
The molecular weight and pI values of the polypeptide chains are consistent with the predicted values and the amino acid compositions similar to predicted sequences.
CDS regions were predicted using ESTScan v2.2.1 (Iseli et al., 1999), and only predicted sequences longer than 50 bp were retained.
Subsequently, these predicted sequences should be validated experimentally through the chemical synthesis of an artificial gene, followed by protein expression and purification.
In those cases predicted sequences were used for alignment purposes.
In those cases predicted sequences were used for alignment, probe generation and sequencing.
Sequencing of the extracted plasmids by Macrogen (Seoul, South Korea) confirmed the predicted sequences of the DNA fragments.
As expected, the three strains had many minor differences in the predicted sequences of the flagellar proteins.
The predicted sequences of the two proteins do not contain any conserved motifs that might indicate their function.
The incorrectly predicted sequences are mostly located in regions in which their phenotype represents the minority or in close vicinity of regions dominated by the opposite phenotype.
The positions of positively charged and hydrophobic amino acids in the predicted sequences matched well with those in the DREAM4 gold standards.
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