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This is in agreement with an earlier finding of Jones and colleagues [ 15], sharing that once the FEV1 falls below 50% of predicted restrictions to essential activities of daily living become more apparent.
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Markers that failed could be due to undetected SNPs in the sequence that modify the predicted restriction sites, or errors in SNP prediction.
This comparison confirmed the predicted restriction fragment lengths of AbSV genomic DNA, as well as its circular topology.
Experimental restriction digests of the MaSp1-positive and MaSp2-positive clones matched predicted restriction sites in the final sequences, verifying that assembly had not erroneously excluded repetitive sequence.
The size of the Tn-containing segments was then compared to the predicted restriction map of the B. subtilis genome using a software developed in the laboratory (available upon request).
If the chromosome is circular then the hybridization products with the probe should be the sum of the sizes of the predicted restriction fragments from each end plus any missing sequence.
The H. mustelae genome also encodes 6 predicted restriction-modification systems.
Reads that did not align to any predicted restriction site were identified as "null".
Paired reads with one end aligning to a restriction site and the other end aligning to no predicted restriction site were designated "singlets".
Methylation sensitivity was determined by comparing nucleotide frequencies around the set of total, predicted restriction sites to nucleotide frequencies around predicted sites with sequencing coverage.
Southern blots using different probes (data not shown) further confirmed this by revealing the bands expected based on the predicted restriction map.
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