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When aligned to the Oryza sativa, reference genome via available BES, the selected MTP spanned over 19.4 Mb of the reference genome with an average predicted clone size of 159 kb, and an average predicted overlap between BACs of 42 kb.
We did not use the later modification of predicted overlap by Nunn et al. [85] that takes into account the number of cycles to conception and non-fertile matings because high-quality data were not available for coding all the species.
* No target gene (validated or predicted) overlap.
The minimal predicted overlap between the E09 epitope and that of FasL, suggested that E09 was not functioning as a direct ligand mimetic.
Previous analysis of sRNA expression in a GAS M1T1 serotype using an intergenic tiling array approach identified 40 potential sRNAs, with a very low predicted overlap with candidate genes [ 20].
In line with the predicted overlap of p53-bound sites and Sp1 RE, the binding of Sp1 to all genes was significantly increased after RITA treatment, suggesting a recruitment of Sp1 upon p53 activation.
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The overlapping score OS Pc, Kc) between a predicted overlapping structure Pc and a known complex Kc is calculated by the following formula: (4) where i is the size of the interaction set of the predicted overlapping structure and the known complex, |V Pc| is the size of the predicted overlapping structure and | V Kc| is the size of the known complex.
The problem is illustrated by the performance of the ab initio versions of ExAlt, which correctly predicted overlapping exons, but at the expense of increased over prediction of splice sites.
In the case of clone F4, there were two predicted overlapping cellulase ORFs (F4_1 and F4_2), both closely related to Pyrococcus spp. (Additional file 1: Table S1).
A known complex Kc that has no proteins in a predicted overlapping structure Pc has OS Pc, Kc) = 0 and a known complex Kc that perfectly matches a predicted overlapping structure Pc has OS Pc, Kc) = 1.
With the exception of the PGRP domain, the vast majority of unigenes also contain a predicted overlapped amidase domain at their C-terminus.
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