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Separate validation experiment at predicted optimal condition ascertained the high predictive ability of the model equation.
An experiment was performed under the predicted optimal conditions in order to validate the optimized medium.
To verify the optimization results, experiments were performed under the predicted optimal condition.
At RSM predicted optimal condition viz.
An additional validation run was adopted using the predicted optimal medium with predicted optimal conditions.
The predicted optimal setting given from this table is A3B1C3.
The predicted optimal hyperthermophilic esterase yield was 269.
These predicted optimal conditions agreed well with the experimental results.
Under these conditions the predicted optimal yield was 52.33 mg.
This may results in erroneous estimation of uncertainty in the predicted optimal reservoir production performance.
Predicted optimal conditions corresponding to two different optimal resolution regions were verified experimentally.
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