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Limitation may operate even at the intracellular level, whereby temporary nutrient limitation within cells due to the demands of protein expression results in predictable changes in composition among proteins with predictable expression profiles such as nutrient assimilation proteins [ 1], differentially expressed protein variants [ 6], or according to transient expression profiles [ 13].
Ex vivo expansion of NK cells results not only in increased amount of cells but also in a consistently superior and predictable expression of NKG2D, NKp30 and NKp44.
The results presented here highlight that the combination of three independent tools/methodologies facilitate the reliable installation of synthetic gene circuits with predictable expression characteristics in mammalian cells and organisms.
Importantly, as a genomic DNA fragment with effective insulator characteristics, BAC vectors enable the generation of transgenic mammalian cells with predictable expression characteristics independent of the integration site, so they are currently often used in genetic research, such as library construction, transgenic mice production, and gene targeting constructs [20], [21].
Upon considering the specific requirements of chromosomal sites this method provides a unique tool to exploit such sites for predictable expression of biotechnologically relevant proteins such as antibodies.
Thereby, this method provides a unique tool to exploit such sites for predictable expression of biotechnologically relevant proteins such as antibodies.
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To date, beyond the predictable expressions of anguish and indignation, I have seen little evidence that the EU institutions appreciate what is going on.
But it has been greeted with predictable expressions of despair.In Indonesia, for instance, only 15% of respondents had a favourable opinion of America: a dramatic reversal from a year ago, when more than 60% viewed America positively.
In transgenic plants, it is difficult to achieve stable and predictable transgene expression over subsequent generations.
This review discusses the current challenges to rendering culturable, non-model members of microbial communities genetically tractable – including overcoming barriers to DNA delivery, achieving predictable gene expression, and applying CRISPR-based tools – and details recent efforts to create generalized pipelines that simplify and expedite the tool-development process.
From a CDC viewpoint, however, this challenge is instead an advantage, since it allows a predictable constituent expression in the systems.
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