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However, in study 1 cattle were culled at a predetermined time point post challenge or, in both studies, at a predetermined end point when the development of BSE was not expected in surviving animals.
As shown in Fig. 8, the DNA percent content at the predetermined time point maintained above 95% during 20 days.
The cells (3 × 105) were seeded on the scaffold which is immersed in the media and allowed to incubate for a predetermined time point.
Myofibroblasts were collected from the gels at the predetermined time point using collagenase 5 mg/ml (Sigma, St . Louis MO) containing cycloheximide (100 µg/ml) and collected by centrifugation.
At the predetermined time point, an aliquot of 20 μl volumes were removed from each test suspension and plated on SDA for determining the colony forming units.
Then, at each predetermined time point (0, 4, 8, 24, 48, and 72 h after drug administration), six mice were sacrificed.
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At predetermined time points, aliquots were withdrawn from the beaker and replaced with equal volume of the media.
At predetermined time points, serial blood samples for cortisol and salicylate concentrations, electrodermal activity measurements, heart rates, and pressure mat data were collected.
The ratios in group IIb (successful expectant management) were compared to the ratios in the group IIa (unsuccessful expectant management) at predetermined time points.
The extent of growth inhibition was measured after predetermined time points of 3, 6, 12, 24, and 48 h by performing MTT assay.
At predetermined time points, 5.0 mL aliquots were withdrawn from the dissolution medium and replaced with fresh medium to maintain a constant volume.
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