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This is because the internal oligonucleotides have predetermined mutations, thus all specific PCR fragments contain the mutations after PCR.
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This assay determines mutation status using a predetermined cutoff ΔCt value (Ct [allele-specific assay] – Ct [reference assay]) as described [ 10].
Here we simulated epistatic datasets which are not constrained to specific predetermined models but instead achieve a diversity of gene-disease relationships created by the randomness of mutation and enhanced through the model-agnostic selective power of evolution.
Nothing is predetermined.
Its decision was predetermined.
Are events predetermined?
That is predetermined.
My fate is predetermined.
"It is all predetermined.
It is predetermined".
The outcome is predetermined.
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