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This is because the internal oligonucleotides have predetermined mutations, thus all specific PCR fragments contain the mutations after PCR.
Significantly mutated genes (SMGs) were defined as the somatic mutations with higher mutated frequency than background mutation rate.
Mutation rate: mutations per cell per generation.
Lynch, M. Evolution of the mutation rate.
fg=3.9 A gene-specific background mutation rate estimated by MutSigCV.
We used a grass-specific mutation rate of 1 × 10−8.
(b) Region with increased maternal mutation rate on chromosome 9 (chr9: 0-10,000,000).
The mutation rate was set to 2 × 10−8.
Genomic factors that affect mutation rate in non-coding elements.
(c) Region with increased maternal mutation rate on chromosome 9 (chr9: 0-10,000,000).
(d) Region with increased maternal mutation rate on chromosome 2 (chr2: 0-10,000,000).
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