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Filter paper discs containing predetermined concentrations of M. persimmonesis were placed on the agar surface.
The experimental solutions were made by diluting the stock solution up to the predetermined concentrations.
DNA samples containing predetermined concentrations and ratios of wild-type and mutated plasmids, bacterial artificial chromosomes of interest, and nonhuman genomic carrier DNA were characterized and tested in-house and in a group of nine pilot testing laboratories using a variety of technical platforms.
For each transcript analyzed, a standard curve with predetermined concentrations and serial diluted respective PCR amplification products from 0.1 ng to 0.000 01 ng was constructed.
To quantify the GLP-1C incorporation efficiency, PEG hydrogels containing predetermined concentrations of GLP-1C (5 mm diameter × 1 mm thick) were fabricated as described and incubated in PBS at room temperature for 48 h.
The Fenton process was conducted at room temperature (25 °C) in the batch mode using predetermined concentrations of reagents.
Similar(46)
Drug concentrations were interpolated from a fluorescence calibration curve, based on predetermined drug concentrations using absorption spectroscopy.
Carbon steel samples were each immersed in 200 ml of the acid solutions (1 M HCl and 0.5 M H2SO4) at predetermined molar concentrations of the organic mixture (TPD for 240 h at 25 °C ambient temperature).
Cell surface staining with antibodies against CD3, CD4 and CD8 added in FACS buffer (PBS with 1% BSA and 0.05% sodium azide) at predetermined optimal concentrations was performed before fixation of cells for 15 min with 4% paraformaldehyde.
Cells not of the DC lineage were depleted by incubating in predetermined optimal concentrations of purified Abs: anti-CD3 (KT3), anti-Thy1 (T24/31.7), anti-CD19 (ID3), anti-GR-1 (RB6-8C5), and anti-erythrocyte (TER-119) and then removing the Ab-binding cells with anti-rat Ig-coupled magnetic beads (Dynabeads; Dynal, Oslo, Norway).
After washing the slides twice in PBS, they were stained using predetermined optimal concentrations of primary Abs for 20 min.
Related(20)
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