On Tuesday, she just needed to pay the rest of the predetermined price after 6 a.m.
Rats were sacrificed after predetermined periods; serum levels of bone turnover markers were evaluated.
They predetermined the times after dosing (3, 6, 12, 24, or 32 h), the distribution of radioactivity measured ex vivo on different time points [12].
The extent of growth inhibition was measured after predetermined time points of 3, 6, 12, 24, and 48 h by performing MTT assay.
After predetermined time intervals, media was discarded from cel-seeded scaffolds followed by washing with PBS thrice and incubation with 200 μl of 5 mg ml−1 MTT solution (Sigma) at 37 °C for 4 h.
After predetermined incubation periods, proteolytic activity was blocked by adding 1 µl of PMSF, a serine protease inhibitor, to reach a final concentration of 1 mM.
After predetermined exclusion criteria were applied, data on 3439 patients were entered into a multivariate analysis.
Following surgery, mice were sacrificed, and livers and caval blood samples were collected at predetermined time points after operation.
After predetermined contact time, the foam can be removed from the site with a sterile cloth thus making the site ready to be operated on.
