At the final follow-up and according to a predesigned scoring system, there were 7 35%) excellent results, 6 30%) good results, 7 35%) fair results, and no poor results.
Quantitative RT-PCR analysis was performed using a StepOnePlus system, predesigned TaqMan gene expression assays, and TaqMan gene expression mastermix (Applied Biosystems).
Real-time PCR was performed on an ABI 7300 real-time PCR machine (Applied Biosystems) using the TaqMan gene expression system (Applied Biosystems) and predesigned fluorescein-labeled primer/probes (β-actin, IL-6: Mm00446190_m1; IL-10: Mm00439614_m1; TNF-α: Mm00443258_m1; IL-1β: Mm00434228_m1; IL-12b: Mm00434174_m1; Life Technologies).
The displacement probes tested produced sensitive and efficient quantitative analyses of template serial dilutions when compared with a range of commercially available predesigned real-time PCR detection systems, including TaqMan MGB probes, QuantiTect MGB probes, and LUX primers.
We consider a system for which two predesigned stabilizing output feedback controllers with bounded domains of attraction are known.
Figure 5 presents the change in outage probability of DSSC full-duplex systems in terms of predesigned threshold κ when the DF protocol is used at the selected relay.
Data were retrieved using a predesigned proforma and entered into the database system for analysis.
mRNA expression was measured on an ABIPRISM 7000 Sequence Detection System (Life Technologies Ltd ,Paisley, U.K). using predesigned Taqman® Gene Expression Assays for RAB25 (Hs00220628_m1) and HPRT1 (Hs99999909_m1) (Life Technologies Ltd ,Paisley, U.K ., as previously described [ 32].
We focus here on periodic communication schedules in control systems with time-triggered control communication and assume the existence of predesigned controllers which need to be optimally integrated via the control communication system.
Similar to the case of AF, the system outage probability decreases significantly when the predesigned threshold κ increases and obtains the lowest value when κ=γ th=7, which verifies our formulation in Section 3.
Next, quantitative RT-PCR was performed using an ABI 7300 system and TaqMan one-step Master Mix kit and predesigned primer/probe pairs for AMPKα1, AMPKα2, AMPKβ1, AMPKβ2, AMPKγ1 AMPKγ3, AMPKγ3 LKB1, HNF4α and β2-microglobulin (Applied Biosystems).
