Thermogravimetric analysis (TGA) and differential thermal analysis (DTA) were used to understand the decomposition profile of the precursors and to select pyrolysis temperatures located in regions of thermal stability.
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A quadrupole analyzer was used to select precursor ions for fragmentation in the hexapole collision cell.
Scores are used to select precursors with highest probability of being genuine (the program kept precursors with scores higher than −50).
In targeted analysis with a Q-TOF instrument, MS1 may be set to select particular precursor ions in prespecified time segments of the chromatogram (time-scheduled product-ion MS/MS).
In the MS/MS mode, the quadrupole was used to select the precursor ions, which were fragmented in the hexapole collision cell generating product ions that were subsequently mass analyzed by the orthogonal reflectron TOF mass analyzer.
A quadrupole mass filter enables the user to select the desired precursor ion (H3O+, NO+, or O2+).
This work developed a method to select the superior precursors instantaneously using molecular dynamic simulation.
Dynamic exclusion of a precursor selected for MS2 was used for 30s after one repeat, enabling most of the co-eluting precursors to be selected for MS2.
Identified peptides unique to G protein isoforms were selected, and their MS/MS spectra were examined to select precursor product ion transitions for targeted MRM experiments.
